Sunday, October 18, 2009

Rapid test for Dengue Virus,

Dengue virus is a mosquito-borne flavivirus and as everyone knows rapid detection and diagnosis is important. Rapid test kits are being introduced as it is able to detect and provide the result faster than routine laboratory methods like ELISA or Haemagglutination inhibition test.

Dengue Virus IgM / IgG antibody Rapid test kit.



Procedure.

  1. pipette 10 ul of patient serum into the square well
  2. drop 3 - 4 drops of test buffer into the round well
  3. start timer ( 15-20 mins )
  4. read results at 15 - 20mins
  5. results should not be read after 20 mins as false results might be obtained.

Reading of Results.

Purple or blue line should be observed at the C - control


If no line is observed, the test is invalid and have to be repeated.

Positive - purple or blue line observed at either M ( IgM ) or G ( IgG ) and C ( control )


Negative - only control line is observed.


Interpretation.


Positive IgM suggest primary or recent dengue infection.


Positive IgM &/or IgG suggest secondary or subsequent dengue infections.




Kenneth.
TG02
Posted by at

2 comments:

  1. TG01-Group 2October 23, 2009 at 10:25 PM

    Hi kenneth,

    you mentioned that this rapid test kit is faster than the other routine lab tests.

    what is the principle behind this test?

    JIAHUI
    tg01 0703605f group 2

    ReplyDelete
  2. MedTech JrOctober 25, 2009 at 7:09 AM

    hello JIAHUI,

    this test is a immunochromatographic assay. it is designed to detect IgM and IgG simultaneously in serum.
    Each test kit has three pre-coated lines on the strip membrane which contains anti-human antibodies specific to dengue IgM and IgG.
    the samples are added to the well followed by buffer causing the sample and antibody-gold conjugate in the second well to migrate upwards. As the samples migrate across the membrane, the anti-dengue human IgM and IgG will be captured and generate coloured lines.

    ReplyDelete

Subscribe to: Post Comments (Atom)