Hello Wess,
sorry for not being very clear. The numbers are the values obtain when the absorbance is measured at 450nm.
And it when the control is 0.350, whether its the positive control or the negative control, the run has to be rejected and troubleshooting must be carried out.
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Hello Siti,
for the negative results its less than 0.85 x COV, sorry for the typo.
as for false positive, yes it is possible as VZV IgG may crossreact with other antigens found in Herpes viridae species. And to confirm, VZV IgM immunofluorescence assay (IF) can be carried out to further confirm the diagnosis.
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Hello YinLiang,
sorry for not stating, the absorbance used for reading is 450nm.
And DUHHHH, other viruses that can be detected using ELISA is dengue virus and rubella virus.
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thanks,
kenneth
Sunday, July 19, 2009
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Ohhh..okay i understand now..
ReplyDeleteThanks Kenneth!!
=)
Siti
tg02
Hello Kenneth,
ReplyDeleteOther than ELISA, what other assay can be use to detect VZV (varicella-zoster virus)?
Hello Dr Alex Lee,
ReplyDeletesorry for the late reply, other than ELISA, immunoflourescence, Polymerase chain reaction and isolation/tissue culture are some of the tests that are able to detect VZV.